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Less common "doping" agents and substances encountered during routine screening for drugs
British Journal of Sports Medicine
|October 1, 1976
Summary
Researchers analyzed racehorse urine and saliva, identifying unusual substances using chromatography and mass spectrometry. Some compounds were linked to sample degradation, suggesting putrefaction in aged samples.
Area of Science:
- Analytical Chemistry
- Forensic Science
- Equine Science
Background:
- Racehorses produce unique biological samples (urine, saliva) that can contain various compounds.
- Understanding the chemical composition of these samples is crucial for performance monitoring and doping control.
Purpose of the Study:
- To characterize unusual substances found in racehorse urine and saliva extracts.
- To identify the source of detected compounds and investigate unknown substances.
Main Methods:
- Extraction of "alkaloidal" substances using chloroform.
- Chromatographic techniques for separation and analysis.
- Spectroscopic methods, including combined gas chromatography-mass spectrometry (GC-MS), for identification.
Main Results:
- Several unusual substances were detected in racehorse urine and saliva.
- Some substances were identified, with their sources noted where known.
- Unidentified substances with characteristic amine fragments were observed, particularly in aged urine samples.
Conclusions:
- The study reports the characterization of novel compounds in equine samples.
- Unidentified substances appear to be associated with sample putrefaction, especially in aged urine.
- Further investigation is needed to fully elucidate the identity and origin of all detected compounds.
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