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A/JAX inbred mice in chemotherapeutic investigations of experimental Chagas' disease
Annals of Tropical Medicine and Parasitology
|August 1, 1978
Abstract:
A quick, simple and relatively inexpensive primary screen to test compounds for activity in experimental Chagas' disease is described. It involves administration of drug once only at the maximum tolerated dose five minutes after infection to a group of five A/JAX inbred mice and the determination of the time to death. The validity of the method was established by the use of standard drugs. High levels of activity were detected with bisquinaldines, arsenobenzenes, 5-nitrofurans and 2-nitroimidazoles.
Insights
A new, cost-effective method screens compounds for Chagas
Area of Science:
- Parasitology
- Drug Discovery
- Tropical Diseases
Background:
- Chagas' disease poses a significant global health challenge.
- Developing effective treatments requires efficient screening methods.
- Existing screening protocols can be time-consuming and expensive.
Purpose of the Study:
- To describe a novel, rapid, and economical primary screening assay for identifying anti-Chagas' disease compounds.
- To validate this new assay using established therapeutic agents.
Main Methods:
- A single-dose administration of compounds at the maximum tolerated dose was given to mice 5 minutes post-infection.
- The primary endpoint was the determination of time to death in infected mice.
- A/JAX inbred mice were utilized for the experimental model.
Main Results:
- The assay demonstrated validity through successful use of standard anti-Chagas' drugs.
- Significant compound activity was identified within specific chemical classes.
- Effective compound classes included bisquinaldines, arsenobenzenes, 5-nitrofurans, and 2-nitroimidazoles.
Conclusions:
- The described method offers a quick, simple, and cost-effective primary screen for experimental Chagas' disease.
- This assay can efficiently detect activity in promising compound classes for Chagas' disease drug development.