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Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation
Published on: June 4, 2015
The occurence and significance of microaggregates in stored blood
Abstract:
During storage whole blood progressively forms microaggregates by adhesion of platelets and leucocytes. This occurs from the second day of storage and the presence of such potential microemboli can be demonstrated by light microscopy of smears of blood samples. They cause an increase in the viscosity of the blood as measurable by the screen filtration pressure method. If infused into a patient they, by causing microvascular occlusion, result in multiple organ dysfunction, primarily of the lung. It is recommended that, if whole blood greater than 4 days in storage is to be infused, an in-line microfilter be used to remove the microaggregates from the transfusion. Alternatively, early separation, or the use of fresh blood is recommended.
Insights
Stored whole blood forms microaggregates, increasing blood viscosity and risking organ damage. Using microfilters for blood stored over 4 days or opting for fresh blood can prevent transfusion complications.
Area of Science:
- Hematology
- Transfusion Medicine
- Pathology
Background:
- Whole blood undergoes changes during storage, leading to the formation of microaggregates.
- These microaggregates are composed of adhered platelets and leukocytes, appearing from the second day of storage.
- The presence of microaggregates can be detected via light microscopy of blood smears.
Purpose of the Study:
- To investigate the formation and impact of microaggregates in stored whole blood.
- To assess the relationship between microaggregate formation, blood viscosity, and potential patient harm.
- To recommend strategies for mitigating transfusion-related complications caused by microaggregates.
Main Methods:
- Light microscopy was used to visualize microaggregate formation in stored blood samples.
- Screen filtration pressure method was employed to measure changes in blood viscosity.
- Clinical implications of microaggregate infusion were analyzed.
Main Results:
- Microaggregate formation begins by the second day of whole blood storage.
- Increased blood viscosity correlates with the presence of microaggregates.
- Infusion of stored blood containing microaggregates can lead to microvascular occlusion and multiple organ dysfunction, particularly affecting the lungs.
Conclusions:
- Whole blood stored for more than 4 days poses a risk of microaggregate-induced complications.
- Utilizing in-line microfilters during transfusion of stored whole blood is recommended.
- Alternative strategies include early separation of blood components or the use of fresh blood to minimize risks.
