The assay of clostridial vaccines in mice

The proposed establishment of a multicomponent clostridial standard vaccine inevitably raises the question of how such a preparation might be used in the standardization of batches of vaccine. Traditional multipoint parallel line assays based on the antitoxin responses of rabbits and guinea pigs are likely to prove prohibitively costly, whilst simpler methods based on single dose levels of either vaccine fail to quantitate the relationship between test vaccine and standard.