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Related Experiment Videos

Predicting insecticide resistance: mutagenesis, selection and response.

J A McKenzie1, P Batterham

  • 1Department of Genetics, University of Melbourne, Parkville, Victoria, Australia. j.mc.kenzie@genetics.unimelb.edu.au

Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
|February 18, 1999
PubMed
Summary

Predicting insecticide resistance mechanisms in the Australian sheep blowfly (Lucilia cuprina) is possible. Laboratory studies identified low-level resistance to cyromazine, explaining field susceptibility.

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Area of Science:

  • Entomology
  • Pest Management
  • Molecular Biology

Background:

  • Insecticide resistance management strategies are typically reactive.
  • Predicting resistance mechanisms could enable proactive susceptibility management programs.

Purpose of the Study:

  • To investigate the potential for predicting insecticide resistance mechanisms.
  • To explore the reasons for sustained susceptibility to cyromazine in Lucilia cuprina populations.

Main Methods:

  • Laboratory mutagenesis of susceptible Australian sheep blowfly (Lucilia cuprina) strains.
  • Selection of single-gene variants resistant to dieldrin, diazinon, and malathion.
  • Analysis of genetic and molecular bases of resistance.
  • Generation and analysis of laboratory-resistant variants to cyromazine.

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Main Results:

  • Laboratory-selected resistance mechanisms mirrored those evolved in natural populations for dieldrin, diazinon, and malathion.
  • Laboratory-generated cyromazine-resistant variants exhibited low resistance levels.
  • These variants showed a selective advantage over susceptible populations only within a narrow concentration range.

Conclusions:

  • The study demonstrates the feasibility of predicting insecticide resistance mechanisms.
  • Low resistance levels and limited selective advantage may explain the sustained field susceptibility to cyromazine.
  • Findings inform insecticide selection and delivery strategies to mitigate resistance evolution.