Nuclease resistant hammerhead motif: from '5-ribo' to '3-ribo' model
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Updated: Oct 24, 2026

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
Published on: July 8, 2019
J Matulic-Adamic1, A T Daniher, C Gonzalez
1Department of Chemistry & Biochemistry, Ribozyme Pharmaceuticals, Inc., Boulder, CO 80301, USA.
Abstract:
Previously developed '5-ribo' nuclease stabilized hammerhead motif was further refined by systematic incorporation of 1-(beta-D-xylofuranosyl) adenine (xA) and 1-(beta-D-xylofuranosyl) guanine (xG) in the place of conserved ribopurine residues of the catalytic core. Modified ribozymes substituted with xA at positions A15.1 and A6 demonstrated catalytic activity close to the parent stabilized ribozyme. Analogous guanosine substitutions at positions G5, G8, and G12 substantially lowered catalytic rates.
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