Synthesis of 5-(2-amino-2-deoxy-beta-D-glucopyranosyloxymethyl)-2'- deoxyuridine and its incorporation into
1Department of Chemistry and Biochemistry, University of Bern, Switzerland. hunziker@ioc.unibe.ch
Abstract:
The protected 5-(2-amino-2-deoxy-beta-D-glucopyranosyloxymethyl)-2'- deoxyuridine phosphoramidite 15 has been prepared from uridine in 12 steps. When incorporated into oligodeoxyribonucleotides the novel nucleoside analog 5 leads to decreased binding affinity. This decrease is larger with a complementary RNA-strand than with a DNA complement.
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