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Related Experiment Videos

The mouse cornichon gene family.

S Y Hwang1, B Oh, Z Zhang

  • 1The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.

Development Genes and Evolution
|February 18, 1999
PubMed
Summary

Researchers identified a mouse cornichon homolog (Cnih) as a maternal transcript crucial for early mammalian development. This finding offers new insights into epidermal growth factor (EGF) signaling in oocytes.

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Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Reproductive Biology

Background:

  • Mammalian development initiation involves identifying key molecular players.
  • The Drosophila cornichon gene is vital for establishing embryonic polarity.
  • Epidermal growth factor (EGF) receptor signaling is critical in developmental processes.

Purpose of the Study:

  • To identify novel mouse genes involved in early mammalian development.
  • To characterize a newly discovered mouse cornichon homolog (Cnih).
  • To investigate the role and expression pattern of Cnih in oocytes and early embryos.

Main Methods:

  • Expressed Sequence Tag (EST) sequencing in mice.
  • Gene mapping to identify chromosomal locations of cornichon homologs.

Related Experiment Videos

  • Analysis of open reading frame (ORF) homology.
  • Quantitative analysis of transcript abundance (Northern blot implied).
  • In situ hybridization for transcript localization.
  • Assessment of mRNA polyadenylation status.

    • Main Results:

    • A mouse maternal transcript, Cnih, homologous to Drosophila cornichon, was identified.
    • Cnih maps to chromosome 10; another homolog, Cnil, maps to chromosome 14.
    • Cnih shares high homology with the Drosophila protein, while Cnil has unique regions.
    • Cnih transcripts are abundant in oocytes and unfertilized eggs, localized specifically to ovarian oocytes.
    • Cnil transcripts appear post-embryonic genome activation (eight-cell stage).
    • Cnih mRNA lacks cytoplasmic polyadenylation, suggesting translation before ovulation.

    Conclusions:

    • Cnih is a maternally inherited transcript expressed in mouse oocytes.
    • The expression pattern suggests a role for Cnih in oocyte maturation or early development.
    • The presence of a cornichon homolog in mammalian oocytes opens new avenues for studying EGF-signaling pathways in reproduction.