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Non-flammable preparative reversed-phase liquid chromatography of recombinant human insulin-like growth factor-I
R L Fahrner1, P M Lester, G S Blank
1Department of Recovery Sciences, Genentech, Inc., South San Francisco, CA 94080, USA. fahrner.robert@gene.com
Abstract:
Acetonitrile is used as an eluent for reversed-phase chromatography. However, because it is a flammable solvent, using acetonitrile on a large scale requires expensive equipment and facilities specially designed for flammable solvents. Using a non-flammable solvent as an eluent eliminates this expense. A method was developed to purify recombinant human insulin-like growth factor I by reversed-phase high-performance liquid chromatography using gradient elution with hexylene glycol, a non-flammable replacement for acetonitrile. The separation produced equivalent yield, purity and throughput as reversed-phase chromatography using elution with acetonitrile.
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