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[Isoelectric focusing of an alkylating 3H-cyclophosphamide derivative from human bladder urine]

V Wetzel, E Schaumlöffel

International Journal of Clinical Pharmacology and Biopharmacy

|December 1, 1976

Abstract:

1) A stable polar 3H-cyclophosphamide metabolite capable of alkylating 4-(p-nitrobenzyl) pyridine and relatively high concentrated in human bladder urine was isolated. The compound has the pI value 9.06. It is a phosphoramide mustard conjugate. 2) Determination of the isoelectric point was accomplished by means of a new thin-layer isoelectric focusing technique applicable for characterizing loaded parts of low molecular weight substance mixtures.

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