Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteoglycan involvement in polyamine uptake.

M Belting1, S Persson, L A Fransson

  • 1Department of Cell and Molecular Biology, Section of Cell and Matrix Biology, Lund University, P.O. Box 94, S-221 00 Lund, Sweden. Mattias.Belting@medkem.lu.se

The Biochemical Journal
|February 20, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hypertension and dyslipidemia in women with PCOS: a population-based multiregister study in Sweden.

Human reproduction (Oxford, England)·2026
Same author

Molecular diagnostics of <i>Salmonella</i> and <i>Campylobacter</i> in human/animal fecal samples remain feasible after long-term sample storage without specific requirements.

AIMS microbiology·2022
Same author

Early initiation of anti-androgen treatment is associated with increased probability of spontaneous conception leading to childbirth in women with polycystic ovary syndrome: a population-based multiregistry cohort study in Sweden.

Human reproduction (Oxford, England)·2021
Same author

Fecundity among women with polycystic ovary syndrome (PCOS)-a population-based study.

Human reproduction (Oxford, England)·2019
Same author

Low-molecular-weight heparin adherence and effects on survival within a randomised phase III lung cancer trial (RASTEN).

European journal of cancer (Oxford, England : 1990)·2019
Same author

The pleiotropic role of proteoglycans in extracellular vesicle mediated communication in the tumor microenvironment.

Seminars in cancer biology·2019
Same journal

Mechanistic insights into acetylated histone recognition by the CECR2 bromodomain.

The Biochemical journal·2026
Same journal

Nanobodies against Plasmodium adhesins that block receptor engagement and malaria parasite invasion.

The Biochemical journal·2026
Same journal

Persistence without turnover: the RhoG G12E mutant highlights the role of nucleotide cycling in RhoG signaling.

The Biochemical journal·2026
Same journal

Alternative Splicing of Rice Chloroplastic CuZn Superoxide Dismutase, OsCSD2: Impact on expression and protein characteristics.

The Biochemical journal·2026
Same journal

Difference and similarity between the ubiquitous secretory pathway Ca2+-ATPases, SERCA2b, and SPCA1a.

The Biochemical journal·2026
Same journal

A molecular perspective on dimethylarginine dimethylaminohydrolases structure and function.

The Biochemical journal·2026
See all related articles

Proteoglycans, particularly heparan sulphate proteoglycans, play a key role in spermine uptake by human lung fibroblasts. This finding suggests potential therapeutic strategies targeting proteoglycans for cell growth regulation.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Background:

  • Proteoglycans are complex macromolecules involved in various cellular processes.
  • Spermine is a polyamine crucial for cell growth and proliferation.
  • The specific mechanisms of spermine uptake by human lung fibroblasts are not fully understood.

Purpose of the Study:

  • To investigate the role of proteoglycans in spermine uptake by human lung fibroblasts.
  • To identify specific types of proteoglycans involved in this process.
  • To explore potential therapeutic implications of modulating spermine uptake.

Main Methods:

  • Competitive inhibition assays using exogenous glycosaminoglycans.
  • Enzymatic degradation of proteoglycans using specific lyases.

Related Experiment Videos

  • Chemical treatments to inhibit polyamine biosynthesis and proteoglycan production.
  • Analysis of spermine uptake in wild-type and mutant cell lines (pgsD-677).

    • Main Results:

    • Exogenous heparan sulphate competitively inhibited spermine uptake with high affinity (Ki=0.16+/-0.04 microM).
    • Treatments reducing heparan sulphate proteoglycan levels (heparan sulphate lyase, xyloside, chlorate) significantly decreased spermine uptake.
    • Inhibition of polyamine biosynthesis increased cell-associated heparan sulphate proteoglycans with higher spermine affinity.
    • Mutant cells deficient in heparan sulphate biosynthesis showed reduced, but not abolished, spermine uptake, with chondroitin/dermatan sulphate proteoglycans playing a role.
    • Heparan sulphate or beta-D-xyloside inhibited the growth of fibroblasts dependent on exogenous spermine.

    Conclusions:

    • Heparan sulphate proteoglycans are specifically involved in mediating spermine uptake by human lung fibroblasts.
    • Chondroitin/dermatan sulphate proteoglycans may also contribute to spermine uptake, particularly in cells with impaired heparan sulphate synthesis.
    • Modulation of proteoglycan-mediated spermine uptake, via heparan sulphate or beta-D-xyloside, can inhibit fibroblast proliferation, suggesting potential therapeutic applications.