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  11. Sulphation And Secretion Of The Predominant Secretory Human Colonic Mucin Muc2 In Ulcerative Colitis.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Sulphation And Secretion Of The Predominant Secretory Human Colonic Mucin Muc2 In Ulcerative Colitis.

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Sulphation and secretion of the predominant secretory human colonic mucin MUC2 in ulcerative colitis.

B J Van Klinken1, J W Van der Wal, A W Einerhand

  • 1Pediatric Gastroenterology and Nutrition, Department of Pediatrics, University of Amsterdam, Academic Medical Centre, Amsterdam, The Netherlands.

Gut
|February 23, 1999

View abstract on PubMed

Summary
This summary is machine-generated.

During active ulcerative colitis, MUC2 mucin synthesis decreases, leading to reduced secretion. Compensatory mechanisms maintain the sulphate content of secreted MUC2 despite lower incorporation.

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Area of Science:

  • Gastroenterology
  • Mucosal immunology
  • Proteoglycan research

Background:

  • Ulcerative colitis is characterized by decreased synthesis of the primary human colonic mucin, MUC2.
  • Mucosal protection is compromised in ulcerative colitis, potentially due to alterations in mucin properties.

Purpose of the Study:

  • To investigate potential changes in mucin sulphation and secretion in ulcerative colitis.
  • To understand the mechanisms behind reduced mucosal protection in this condition.

Main Methods:

  • Colonic biopsy specimens from ulcerative colitis patients (active and remission) and controls were analyzed.
  • Metabolic labeling with [35S]-amino acids or [35S]-sulphate followed by SDS-PAGE and dot blotting quantified MUC2.
  • [35S]-sulphate incorporation and secretion of MUC2 were measured.

Main Results:

  • No significant differences in the [35S]-sulphate content of secreted MUC2 or the secreted percentage of MUC2 were observed between groups.
  • Secretion of [35S]-sulphate labeled MUC2 increased twofold during active ulcerative colitis.
  • [35S]-sulphate incorporation into MUC2 was halved during active ulcerative colitis.

Conclusions:

  • Decreased MUC2 synthesis, not altered secretion percentage, causes reduced MUC2 secretion in active ulcerative colitis.
  • A compensatory mechanism maintains the sulphate content of secreted MUC2.
  • Preferential secretion of sulphated MUC2 occurs despite reduced sulphate incorporation.