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Sandwich enzyme-linked immunosorbent assay by using monoclonal antibody for detection of Clostridium perfringens
R H Piyankarage1, T Tajima, S Sugii
1Laboratories of Veterinary Public Health, Osaka Prefecture University, Japan.
The Journal of Veterinary Medical Science
|February 23, 1999
Abstract:
Sandwich enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative estimation of Clostridium perfringens enterotoxin (CPE) with monoclonal and polyclonal antibodies as capturing and detecting antibodies, respectively. The dose-dependent relationship between absorbance at 405 nm and concentration of purified CPE was obtained over the range of 0.64-400 ng/ml. The sandwich ELISA was fond to detect crude CPE in culture and CPE in 10% fecal extracts. This method is convenient, rapid and sensitive for specific detection of CPE.