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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
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Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

Published on: December 21, 2010

Generating a bacterial genome inventory. Identifying 2-D spots by comigrating products of the genome on 2-D gels

R A VanBogelen1

  • 1Biotechnology Laboratory, Parke-Davis Pharmaceutical Research, Ann Arbor, MI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 23, 1999
PubMed
Abstract

No abstract available in PubMed .

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...