Inhibition of norepinephrine release in the rat ventromedial hypothalamic nucleus in essential amino acid deficiency

Effects of dietary amino acid deficiency on interstitial levels of norepinephrine (NE) were assessed in the ventromedial hypothalamic nucleus (VMH). Microdialysates, collected from the VMH, were analyzed using high pressure liquid chromatography with electrochemical detection (HPLC-EC). Ingestion of an amino acid imbalanced diet, which causes a rapid deficiency of the limiting amino acid, induced a significant decrease in the NE concentration from the VMH. The changes in the NE concentration appeared 60 min after diet ingestion and the lowest NE level was observed at 180 min. The present results suggest that ingestion of an amino acid imbalanced diet inhibits NE release in the VMH and support the hypothesis that the VMH plays a role in the integration of signals for the feeding responses to changes in essential amino acid availability.