Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Screening nitrate in forages with a test strip: collaborative study.

A V Jain1, P F Ross, M P Carlson

  • 1University of Georgia, College of Veterinary Medicine, Athens Veterinary Diagnostic Laboratory 30602-7383, USA.

Journal of AOAC International
|February 24, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disruption of sphingolipid metabolism and induction of equine leukoencephalomalacia by Fusarium proliferatum culture material containing fumonisin B(2) or B(3).

Environmental toxicology and pharmacology·2011
Same author

Diagnostic implications of antigen-induced gamma interferon, nitric oxide, and tumor necrosis factor alpha production by peripheral blood mononuclear cells from Mycobacterium bovis-infected cattle.

Clinical and diagnostic laboratory immunology·2003
Same author

An international collaborative study on a method for determination of formaldehyde in veterinary vaccines.

Biologicals : journal of the International Association of Biological Standardization·2002
Same author

Minimal prophylactic concentration of dietary zinc compounds in a mouse model of swine dysentery.

Animal health research reviews·2001
Same author

Effects of inorganic and organic copper supplemented to first-calf cows on cow reproduction and calf health and performance.

Journal of animal science·2001
Same author

Earth system science sentiments.

Science (New York, N.Y.)·2001
Same journal

Correction to: Determination of Alkaloids in Mitragyna speciosa (Kratom) Raw Materials and Dietary Supplements by HPLC-UV: Single-Laboratory Validation, First Action 2017.14.

Journal of AOAC International·2026
Same journal

Single-Laboratory Validation of Simultaneous Determination of Aflatoxins in Nutraceuticals following immune-affinity Column Cleanup and Liquid Chromatography Tandem Mass Spectrometry Analysis.

Journal of AOAC International·2026
Same journal

Determination of Bromoform in Seaweed, Oil, and Animal Feed by GC-MS/MS: AOAC Official Method 2026.01, First Action.

Journal of AOAC International·2026
Same journal

ICRF-Assisted Box-Behnken Design and Optimization for Rapid UPLC-PDA Determination of Dorzolamide Hydrochloride and Timolol Maleate in an Ophthalmic Preparation.

Journal of AOAC International·2026
Same journal

Advancing PFAS Analysis Through Scientific Publication.

Journal of AOAC International·2026
Same journal

A Green UPLC-UV Method for L-Ascorbic Acid Determination Based on a Biodegradable Chelating Agent and Synergistic Hydrophobic-Electrostatic Interactions.

Journal of AOAC International·2026
See all related articles

A commercial test strip effectively screens nitrate levels in forages. This method, adopted by AOAC INTERNATIONAL, provides high sensitivity and specificity for accurate nitrate determination in animal feed.

Area of Science:

  • Agricultural Science
  • Analytical Chemistry
  • Food Safety

Background:

  • Nitrate accumulation in forages poses a risk to livestock health.
  • Accurate and accessible screening methods for forage nitrate are essential for animal agriculture.
  • Existing methods can be time-consuming or require specialized equipment.

Purpose of the Study:

  • To evaluate the efficacy of a commercially available test strip for screening nitrate concentrations in forages.
  • To determine the sensitivity, specificity, and accuracy of the test strip method through a collaborative study.
  • To establish the test strip as a reliable first-action method for forage nitrate analysis.

Main Methods:

  • A collaborative study involving 20 laboratories analyzing six blind quintuplicate forage samples.

Related Experiment Videos

  • Samples were extracted with deionized water, and nitrate concentration was determined using a colorimetric test strip.
  • Results were categorized into four nitrate concentration ranges, with statistical analysis including outlier detection.

    • Main Results:

    • The test strip method demonstrated high performance across various nitrate concentrations (<1000 ppm to >10,000 ppm).
    • Sensitivity rates ranged from 0.965 to 0.998, and specificity rates ranged from 0.991 to 0.997.
    • False-positive and false-negative rates were low, indicating reliable screening capabilities.

    Conclusions:

    • The commercially available test strip is a reliable and accurate tool for screening nitrate in forages.
    • The method's ease of use and high accuracy support its adoption as a first-action analytical method.
    • AOAC INTERNATIONAL has adopted this test strip method, validating its utility in agricultural and animal feed safety.