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Thin-layer chromatographic determination of catecholamines, 5-hydroxytryptamine, and their metabolites in biological
G Alemany1, M Akaârir, A Gamundí
1Universitat de les Illes Balears, Department de Biologia Fonamental i Ciències de la Salut, Palma de Mallorca, Spain.
Journal of AOAC International
|February 24, 1999
Abstract:
A review of methodology for separation, detection, and quantitative determination of catecholamines, 5-hydroxytryptamine, and their acidic metabolites in biological tissue and fluids by thin-layer chromatography is presented. Selected procedures, including fluorometric scanning densitometry for catecholamine acetyl derivatives and color scanning densitometry for acids, are described.