[Laboratory diagnosis to dengue virus infections]

Currently, laboratory diagnosis of Dengue virus infection has a special importance because of its emerging character, showing a great geographic propagation of viral activity through out the American Continent. In general, the laboratory diagnosis of Dengue infections is similar to other human infecting viruses, the difference and difficulty of the process lies in the cross reaction activity with other flavivirus that can co-exist in the same region. The effectiveness of the diagnosis depends on the quality of the samples obtained, so it is absolutely necessary to monitor carefully the timing, conditions as well as the accompanying information during the gathering of the samples. The present article describes some of the serologic technics used in the diagnosis of dengue infection: hemaglutination inhibition, plaque reduction neutralization and IgM and IgG ELISA assays, in the same way it outlines the principles and importance of viral isolation and identification as well as the advantages of a new diagnosis technic like PCR.