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Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

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Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection
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Biochemical and cytochemical studies on adenylate cyclase activity in the developing rat submandibular gland:

L S Cutler, S B Rodan

    Journal of Embryology and Experimental Morphology
    |October 1, 1976
    PubMed
    Summary

    Adenylate cyclase activity in rat submandibular glands significantly increases during prenatal development. This enzyme

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    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Biochemistry

    Background:

    • The exocrine cells of the rat submandibular gland (SMG) undergo significant membrane changes during development.
    • Adenylate cyclase is a key enzyme involved in cellular signaling pathways and membrane function.

    Purpose of the Study:

    • To investigate the developmental changes in adenylate cyclase activity and localization within the rat SMG.
    • To correlate biochemical and cytochemical findings of adenylate cyclase during gland development.

    Main Methods:

    • Biochemical assays of adenylate cyclase activity using established procedures.
    • Cytochemical studies to visualize adenylate cyclase localization in prenatal and postnatal SMG.
    • Analysis of glands from 15 days gestation to 24 weeks post-birth.

    Main Results:

    • Basal adenylate cyclase activity was low at 15-16 days gestation.
    • A six-fold increase in adenylate cyclase activity occurred between days 16 and 18 of gestation.
    • Cytochemical staining for adenylate cyclase became evident on cell surfaces by day 18.
    • Activity remained relatively constant postnatally, but localization patterns changed, particularly at the apical membrane.

    Conclusions:

    • Adenylate cyclase activity undergoes a critical surge during late prenatal development of the rat SMG.
    • Cytochemical localization reveals dynamic changes in enzyme distribution, especially at the apical plasma membrane, during secretory cell differentiation.