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Related Experiment Videos

Prothrombin activation in rabbits.

D Seiffert1, T J Mitchell, Z Wang

  • 1Department of Chemical Enzymology, DuPont Pharmaceuticals Company, Wilmington, Delaware, USA. Seiffert@dupont.pharma.com

Thrombosis Research
|February 25, 1999
PubMed
Summary
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Rabbit prothrombin activation fragment F 1.2 is not a reliable marker for coagulation activation. Differences in factor Xa cleavage sites, not cofactors, explain variations in human versus rabbit prothrombin activation.

Area of Science:

  • Biochemistry
  • Hematology
  • Molecular Biology

Background:

  • Prothrombin activation fragment F 1.2 (F 1.2) is a marker for coagulation system activation.
  • Previous studies have primarily focused on human prothrombin activation.

Purpose of the Study:

  • To evaluate rabbit prothrombin activation fragment F 1.2 (F 1.2) as a marker for coagulation activation.
  • To investigate the molecular basis for differences in prothrombin activation between rabbits and humans.

Main Methods:

  • Development of a competitive F 1.2 ELISA using monoclonal antibodies against rabbit F 1.2.
  • Immunoblotting analysis of endogenous and labeled prothrombin.
  • cDNA sequencing of rabbit prothrombin.

Main Results:

Related Experiment Videos

  • Rabbit plasma showed no detectable F 1.2 increase upon recalcification, unlike human plasma.
  • Rabbit serum contained meizothrombin and thrombin B-chain, but not F 1.2, differing from human serum.
  • Rabbit F 1.2 formation was observed with purified components, suggesting altered factor Xa cleavage susceptibility.

Conclusions:

  • Rabbit F 1.2 is not a suitable marker for coagulation activation in rabbits.
  • A six amino acid deletion near the 271/272 site in rabbit prothrombin likely reduces factor Xa cleavage efficiency compared to humans.
  • Differences in prothrombin structure, specifically factor Xa cleavage sites, explain species-specific activation patterns.