Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Filament assembly from profilin-actin.

I Gutsche-Perelroizen1, J Lepault, A Ott

  • 1Laboratoire d'Enzymologie et Biochimie Structurales, CNRS, 91198 Gif-sur-Yvette, France.

The Journal of Biological Chemistry
|February 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ambler class C-type β-lactamases and porin alterations in Enterobacter cloacae complex and Klebsiella aerogenes in the Netherlands, 2012-2023.

BMC microbiology·2026
Same author

CefiderocolFinder: a tool for detecting genetic adaptations implicated in cefiderocol resistance.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2025
Same author

New insight into flavivirus maturation from structure/function studies of the yellow fever virus envelope protein complex.

mBio·2023
Same author

[Electron cryomicroscopy/X-ray crystallography: an approach to study biological processes at atomic resolution].

Virologie (Montrouge, France)·2021
Same author

[Structural relationships among birnaviruses and other icosahedral viruses].

Virologie (Montrouge, France)·2021
Same author

Seroprevalences of antibodies against ToRCH infectious pathogens in women of childbearing age residing in Brazil, Mexico, Germany, Poland, Turkey and China.

Epidemiology and infection·2020

Profilin-actin facilitates filament growth at barbed ends, requiring MgATP and exhibiting slower association rates than actin alone. Cross-linked profilin-actin copolymers mimic actin filament properties but lack motor protein translocation ability.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Biophysics

Background:

  • Profilin is a key regulator of actin filament dynamics.
  • Actin polymerization is crucial for cellular processes.
  • Understanding profilin-actin interactions informs models of cytoskeletal regulation.

Purpose of the Study:

  • To investigate the thermodynamic and kinetic parameters of actin filament assembly from profilin-actin complexes.
  • To characterize the properties of cross-linked profilin-actin polymers.
  • To elucidate the role of profilin in actin dynamics and filament structure.

Main Methods:

  • Turbidimetry to measure thermodynamic and kinetic parameters of actin assembly.
  • Analysis of profilin-actin complex assembly under varying conditions (MgATP vs. CaATP).

Related Experiment Videos

  • Characterization of EDC-cross-linked profilin-actin (PAcov) polymerization and filament properties using diffraction, phalloidin binding, and myosin interactions.

    • Main Results:

    • Profilin-actin assembly requires MgATP, with a 30% lower association rate constant and a critical concentration of 0.3 microM.
    • Filaments grown from profilin-actin possess an ADP-Pi cap and profilin is not incorporated into the filament.
    • PAcov copolymerizes with F-actin and self-assembles with a critical concentration of 0.2 microM, forming helical filaments with actin-like diffraction and binding properties, but impaired motor protein translocation.

    Conclusions:

    • Profilin modulates actin assembly kinetics and thermodynamics without being incorporated into the filament.
    • Cross-linked profilin-actin can form stable filaments that mimic F-actin structure and binding but not motor-driven movement.
    • These findings provide insights into actin structure and the functional consequences of profilin interaction.