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Amplification of cDNA ends based on template-switching effect and step-out PCR
M Matz1, D Shagin, E Bogdanova
1Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117871, Russia.
Nucleic Acids Research
|February 26, 1999
Abstract:
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.