On the transport of various endogenous plasma proteins from blood to peripheral lymph in man

A method to prepare peripheral lymph out of the foot of clinically healthy patients with respect to their blood capillaries and lymph vessels is presented. Following electrophoresis in molecular sieving polyacrylamide gel the lymph/plasma quotients of precentages of identical protein fractions in both body fluids as well as of their concentrations were graphically displayed dependent on their migration velocity within the gel. As the high or low lymph-plasma-relations are necessarily to be interpreted in the sense of a relatively high or low blood-lymph permeation the findings are speaking against an exclusive effect of the molecular sieving principle at the blood-tissue-barrier. An explanation for special lymph-plasma-relations would be provided by the assumption of a gel-filtration effect in the extravascular circuit of plasma-proteins. The results affirm the findings of former experiments on animals in an analogue model. The method as well as simplified variants might be useful for the investigation of peripheral nutritive disorders as well as of clinicopharmacological questionings.