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Heat-sensitive DNA-binding activity of the cI product of bacteriophage lambda
Molecular & General Genetics : MGG
|August 2, 1976
Abstract:
The binding of lambda gene cI product to lambda DNA was studied at temperatures from 0 degrees C to 46 degrees C. Binding activity of the products of cIts mutants was higher at 22 degrees C than at 0 degrees C, 26 degrees C or 30 degrees C. Both cI+ and cIts products lost DNA-binding activity at 46 degrees C, but after subsequent cooling to 22 degrees C, they regained 50-100% of their activity.