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Sequence of metabolic changes during X-ray-induced apoptosis
C Ferlini1, C De Angelis, R Biselli
1Laboratory of Immunology, DASRS, Pratica di Mare, Rome, Italy. ferlini@iol.it
Experimental Cell Research
|February 27, 1999
Summary
Metabolic changes during apoptosis, including cardiolipin alterations and reduced membrane potential, occur early. Oxidative stress and thiol depletion also manifest early, impacting cell function throughout apoptosis.
Area of Science:
- Cell Biology
- Biochemistry
Background:
- Apoptosis involves diverse cell stages, confounding metabolic studies.
- Understanding metabolic shifts during apoptosis is crucial.
Purpose of the Study:
- To investigate metabolic changes during X-ray-induced apoptosis in lymphocytes.
- To correlate metabolic alterations with specific apoptotic stages.
Main Methods:
- Utilized fluorescent probes and multiparameter flow cytometry.
- Assessed apoptotic stages by monitoring plasma membrane permeability.
- Analyzed mitochondrial cardiolipin, plasma membrane potential, and mitochondrial transmembrane potential.
Main Results:
- Early apoptosis shows cardiolipin alterations and reduced plasma membrane potential.
- Mitochondrial transmembrane potential declines with increased membrane permeability.
- Oxidative stress increases, with thiol depletion observed in early apoptosis.
Conclusions:
- Metabolic changes during apoptosis are stage-dependent.
- Oxidative damage is an early event in apoptosis.
- Flow cytometry aids in understanding the temporal sequence of apoptotic events.