Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phage-display technology--finding a needle in a vast molecular haystack.

D J Rodi1, L Makowski

  • 1Institute of Molecular Biophysics Florida State University Tallahassee Florida 32306 USA.

Current Opinion in Biotechnology
|February 27, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sex related differences in therapy and outcome of patients with intermittent claudication in a real-world cohort.

Atherosclerosis·2021
Same author

Lymphedema of the breast as a symptom of internal diseases or side effect of mTor inhibitors.

Lymphatic research and biology·2012
Same author

Multi-wavelength anomalous diffraction using medium-angle X-ray solution scattering (MADMAX).

Biophysical journal·2012
Same author

Characterisation of the learning curve of caesarean section.

Archives of gynecology and obstetrics·2012
Same author

WAXS studies of the structural diversity of hemoglobin in solution.

Journal of molecular biology·2011
Same author

STRUCTURE OF FILAMENTOUS BACTERIOPHAGE Pf1.

Biophysical journal·2009

Phage display screening effectively identifies molecular targets by searching vast libraries. Recent advancements expand its use to find smaller binding motifs and novel chemical activities.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Phage display has been a powerful tool for nearly a decade.
  • It enables the screening of large libraries of proteins and peptides.
  • This technique is crucial for identifying specific molecular interactions.

Purpose of the Study:

  • To highlight the continued effectiveness of phage display screening.
  • To showcase recent advancements and expanded applications of the technique.
  • To emphasize its utility in identifying binding motifs and chemical activities.

Main Methods:

  • Screening of phage-displayed libraries of proteins and peptides.
  • Application of established and novel screening methodologies.
  • Target identification for molecular binding and chemical activity assays.

Related Experiment Videos

Main Results:

  • Phage display remains a highly effective method for identifying specific molecules from large libraries.
  • The technique has been successfully applied to identify binding motifs for small molecular targets.
  • Novel screening approaches have been developed to discover new chemical activities.

Conclusions:

  • Phage display screening is a versatile and robust technology.
  • Its applications continue to expand, addressing diverse challenges in molecular discovery.
  • The method is invaluable for identifying specific molecular interactions and functions.