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Published on: June 6, 2014
Detection of movement at the erythrocyte's edge by scanning phase contrast microscopy
A Bitler1, A Barbul, R Korenstein
1Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel-Aviv, Israel.
Abstract:
A time-dependent set of line scans across an erythrocyte was recorded by a phase contrast laser scanning microscope. A method for edge detection, based on a two-stage fitting procedure of the theoretical intensity distribution in a line scan of phase contrast image to the experimental one, is suggested. Time-resolved fluctuations of the human erythrocyte's edge were obtained, employing this method. The same procedure was carried out for a metallic strip yielding a test for the background noise level. The suggested method was applied in examining the effect of chemical crosslinking and thiol reduction on cell membrane fluctuations of human erythrocytes.
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