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Updated: Aug 14, 2026

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
Published on: January 20, 2014
Histopathological evidence that spermatogonia are the target cells of 2-bromopropane
1Department of Hygiene, Faculty of Medicine, Kyushu University, Fukuoka, Japan. momura@eisei.med.kyushu-u.ac.jp
Abstract:
To confirm the target cell of 2-bromopropane within the testis, 1355 mg/kg of 2-bromopropane was subcutaneously injected to rats for 1-5 days and the numbers of spermatogonia and spermatocytes were examined 6 h after each last injection. The number of stage I spermatogonia decreased after the first 2-bromopropane injection and the number of spermatogonia at the other stages also decreased following repetitive injection. The number of these spermatogonia decreased further by the repetition of 2-bromopropane injection. In addition, the delay in mitotic division of type B spermatogonia was frequently observed after the fifth 2-bromopropane injection. The number of stage I pachytene spermatocytes also decreased slightly after the first 2-bromopropane injection, although it did not decrease further following repetitive injection. Therefore, we concluded that spermatogonia are the target cells of 2-bromopropane in rats.
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