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The effect of volatile anesthetics on light-induced phosphorylation in spinach chloroplasts
1Department of Anesthesiology and Emergency Medicine, Kagawa Medical University, Japan. hiroyuki@kms.ac.jp
Toxicology Letters
|February 27, 1999
Abstract:
(1) The light-induced phosphorylation in spinach chloroplast is coupled with proton transfer. (2) We investigated the effect of volatile anesthetics (halothane, enflurane, isoflurane) on the cyclic and/or non-cyclic light-induced phosphorylation. (3) We used potassium ferricyanide as a Hill oxidant for non-cyclic phosphorylation and phenazin-methosulfate as an oxidation-reduction indicator for cyclic phosphorylation. (4) These three anesthetics inhibit the Hill reaction in light induced phosphorylation. The inhibition rate of this reaction generates concave curves with minimum values at 303 K in each of the anesthetics.