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The porcine hormone-sensitive lipase gene: sequence, structure, polymorphisms and linkage mapping
I Harbitz1, M Langset, A G Ege
1Department of Biochemistry, Physiology and Nutrition, Norwegian School of Veterinary Science, Oslo, Norway.
Animal Genetics
|March 2, 1999
Summary
Researchers isolated and sequenced the porcine hormone-sensitive lipase (LIPE) gene. They identified regulatory sequences and genetic variations, mapping the LIPE gene to chromosome 6.
Area of Science:
- Genetics
- Molecular Biology
- Comparative Genomics
Background:
- The hormone-sensitive lipase (LIPE) is a key enzyme in lipid metabolism.
- Understanding LIPE gene structure and regulation is crucial for metabolic research.
Purpose of the Study:
- To isolate and sequence the porcine LIPE gene and its cDNA.
- To identify regulatory elements and genetic variations within the porcine LIPE gene.
- To determine the chromosomal location of the porcine LIPE gene.
Main Methods:
- Gene isolation and DNA sequencing.
- Bioinformatic analysis of promoter regions.
- Polymorphism screening (intronic and exonic).
- Linkage mapping.
Main Results:
- The porcine LIPE gene and cDNA were successfully isolated and sequenced.
- Several putative regulatory sequences were identified in the promoter region.
- The deduced amino acid sequence showed 85% identity to human, mouse, and rat orthologs.
- One intronic and one exonic polymorphism were found, with the exonic one causing an amino acid substitution.
- Linkage mapping placed the LIPE gene near the calcium release channel (CRC) locus on chromosome 6.
Conclusions:
- The study provides a comprehensive characterization of the porcine LIPE gene.
- Identified polymorphisms may be relevant for future genetic studies in pigs.
- The chromosomal localization offers insights into comparative gene mapping and potential associations with traits regulated by nearby loci.