Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential regulation of uncoupling protein-1, -2 and -3 gene expression by sympathetic innervation in brown

F Denjean1, J Lachuer, A Géloën

  • 1Laboratoire de Physiologie des Régulations Energétiques, Cellulaires et Moléculaires, UMR 5578 CNRS-Université Cl. Bernard Lyon 1, Villeurbanne, France.

FEBS Letters
|March 2, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A phase II, multicenter, open-label, single-arm study of berzosertib plus topotecan in patients with relapsed platinum-resistant small-cell lung cancer (DDRiver SCLC 250).

ESMO open·2025
Same author

Two Rare Complications in One Patient: Acquired von Willebrand Syndrome Associated with Intracranial Plasmacytoma.

Case reports in hematology·2019
Same author

Hypothesis: Fever control, a niche for alpha-2 agonists in the setting of septic shock and severe acute respiratory distress syndrome?

Temperature (Austin, Tex.)·2018
Same author

Metabolic effects in mice of cream processing: Direct ultra-high-temperature process lowers high-fat-induced adipose tissue inflammation.

Journal of dairy science·2018
Same author

Metabolic effects in mice of cream formulation: Addition of both thickener and emulsifier does not alter lipid metabolism but modulates mucus cells and intestinal endoplasmic reticulum stress.

Journal of dairy science·2018
Same author

New insight into the ternary complexes of uranyl carbonate in seawater.

Journal of environmental radioactivity·2017

Sympathetic innervation differentially regulates uncoupling protein (UCP) gene expression in rat brown adipose tissue (BAT). Cold exposure increases UCP-1, UCP-2, and UCP-3 mRNA, but sympathetic denervation only affects UCP-1 and UCP-2.

Area of Science:

  • Physiology
  • Molecular Biology
  • Endocrinology

Background:

  • Uncoupling proteins (UCPs) are crucial for thermogenesis in brown adipose tissue (BAT).
  • Sympathetic nervous system (SNS) activation is a primary regulator of BAT thermogenesis.
  • Differential regulation of UCP-1, UCP-2, and UCP-3 gene expression by the SNS is not fully understood.

Purpose of the Study:

  • To investigate the role of sympathetic innervation in controlling UCP-1, UCP-2, and UCP-3 mRNA levels in rat BAT.
  • To determine if cold exposure and sympathetic denervation differentially affect UCP gene expression.

Main Methods:

  • Specific and sensitive reverse transcription-polymerase chain reaction (RT-PCR) assays were used.
  • Unilateral surgical sympathetic denervation of interscapular BAT was performed in rats.

Related Experiment Videos

  • Northern blot analysis was employed for confirmation.

    • Main Results:

    • At thermoneutrality, sympathetic denervation reduced UCP-1 mRNA by 38% but did not affect UCP-2 or UCP-3 mRNA levels.
    • Cold exposure significantly increased UCP-1, UCP-2, and UCP-3 mRNA levels.
    • Denervation prevented the cold-induced increase in UCP-1 and UCP-2 mRNA but not UCP-3 mRNA.

    Conclusions:

    • Sympathetic innervation exerts differential control over UCP-1, UCP-2, and UCP-3 gene expression in rat BAT.
    • Both thermoneutral conditions and cold exposure reveal distinct regulatory mechanisms for UCP genes by the SNS.