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Concatemerization of tRNA molecules in the presence of trivaline derivative
S A Streltsov1, L P Martinkina, Vengerov YYu
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow. strelcov@imb.imb.ac.ru
Abstract:
The interaction of tRNA with trivaline dansyl hydrazide trifluoroacetate (DHTV) has been studied. The shape of curves of fluorimetric titration of tRNA with DHTV and vice versa can be explained only by formation of DHTV dimers on tRNA molecules, and subsequent association of DHTV-saturated tRNA molecules with each other. The ability of tRNA molecules to form concatemers in solution in the presence of DHTV has been demonstrated by electron microscopy. Electron microscopy of the tRNA-DHTV complexes stained with uranyl acetate revealed flexible rods 6-7 nm thick and up to several micrometers long.