Related Experiment Video
Updated: Jul 11, 2026

High Sensitivity 5-hydroxymethylcytosine Detection in Balb/C Brain Tissue
Published on: February 1, 2011
A mammalian protein with specific demethylase activity for mCpG DNA
S K Bhattacharya1, S Ramchandani, N Cervoni
1Department of Pharmacology and Therapeutics, McGill University, Montreal, QC, Canada.
Abstract:
DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when the cDNA is translated or transiently transfected into human embryonal kidney cells in vitro. The discovery of this DNA demethylase should provide a basis for the molecular and developmental analysis of the role of DNA methylation and demethylation.
More Related Videos
Related Concept Videos
Epigenetic Regulation
Euchromatin
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
The Eukaryotic Promoter Region
Co-activators and Co-repressors
Heterochromatin
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Epigenetic Regulation
X-chromosome...

