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Separation and properties of two acetylacetoin reductases from Bacillus cereus YUF-4
1Department of Applied Chemistry and Biotechnology, Faculty of Engineering Yamanashi University, Japan. g98ds007@ccn.yamanashi.ac.jp
Bioscience, Biotechnology, and Biochemistry
|March 3, 1999
Abstract:
The separation and purification of two kinds of acetylacetoin reductases (AACRs) from Bacillus cereus YUF-4 were examined. NADPH-linked AACR (AACR I) and NADH-linked AACR (AACR II) were separated from each other by ammonium sulfate fractionation, DEAE-cellulose chromatography, and hydroxyapatite chromatography. The former was purified 3.4-fold with a yield of 10.0%, and the latter was purified 29-fold with a yield of 15.6%. The two enzymes differ from each other in some enzymic properties such as substrate specificity.