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Related Experiment Videos

Melatonin inhibits release of luteinizing hormone (LH) via decrease of [Ca2+]i and cyclic AMP.

J Vanecek1

  • 1Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.

Physiological Research
|March 3, 1999
PubMed
Summary

Melatonin inhibits luteinizing hormone (LH) release by decreasing calcium ([Ca2+]i) and cyclic AMP (cAMP). Increasing these messengers counteracted melatonin's effect, suggesting a dual mechanism.

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Area of Science:

  • Endocrinology
  • Neuroendocrinology
  • Cell Signaling

Background:

  • Melatonin is known to inhibit gonadotropin-releasing hormone (GnRH)-induced luteinizing hormone (LH) release.
  • Melatonin's inhibitory mechanism involves the modulation of intracellular calcium ([Ca2+]i) and cyclic adenosine monophosphate (cAMP).

Purpose of the Study:

  • To investigate the roles of [Ca2+]i and cAMP in mediating melatonin's inhibitory effect on GnRH-induced LH release from neonatal rat gonadotrophs.
  • To elucidate the interplay between cAMP and calcium signaling pathways in melatonin's action.

Main Methods:

  • Utilized neonatal rat gonadotrophs as the experimental model.
  • Administered GnRH to stimulate LH release.
  • Applied melatonin and agents that modulate intracellular calcium (e.g., S(-) Bay K8644, KCl) and cAMP (e.g., 8-bromo-cAMP, 3-isobutyl-1-methylxanthine) levels.

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  • Measured intracellular calcium ([Ca2+]i) concentrations and LH release.

    • Main Results:

    • Treatments that increased Ca2+ influx or cAMP concentration potentiated GnRH-induced LH release and partially reduced melatonin's inhibitory effect.
    • Combined elevation of both cAMP and calcium completely blocked melatonin's inhibition of LH release.
    • The combined increase in cAMP and calcium also significantly blocked melatonin's inhibition of GnRH-induced [Ca2+]i increase in gonadotrophs.

    Conclusions:

    • Melatonin's inhibition of GnRH-induced LH release is mediated by a decrease in both intracellular calcium and cAMP levels.
    • A cAMP-dependent pathway is involved in the regulation of calcium influx by melatonin.
    • These findings highlight a complex signaling network involving cAMP and calcium in the neuroendocrine control of LH release by melatonin.