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High-performance liquid chromatographic determination of paclitaxel in rat serum: application to a toxicokinetic
F Coudoré1, N Authier, D Guillaume
1Laboratoire de Toxicologie, Equipe NPPUA, Faculté de Pharmacie, Clermont-Ferrand, France.
Abstract:
A simple and sensitive high-performance liquid chromatographic method is described for the determination of paclitaxel (Taxol) at 230 nm using a Nucleosil C18 (5 microm) column and a methanol-water (70:30, v/v) mobile phase following a single-step extraction from serum with dichloromethane. The assay was validated against the classical criteria and was applied to a toxicokinetic study in rats after one or five, one per week) intraperitoneal administrations of 16 mg/kg Taxol.