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Reverse transcriptase activity and particle production in B lymphoblastoid cell lines established from lymphocytes of
T Christensen1, R R Tönjes, J zur Megede
1Department of Medical Microbiology, University of Aarhus, Denmark. mikrtc@svfcd.aau.dk
AIDS Research and Human Retroviruses
|March 3, 1999
Abstract:
We have established spontaneously formed B lymphoblastoid cell lines from long-term cultured peripheral blood mononuclear cells (PBMNCs) from multiple sclerosis (MS) patients. The MS cell lines actively produce retrovirus-like particles as well as Epstein-Barr virus (EBV). Using three different variations of the highly sensitive polymerase chain reaction (PCR)-based assays for the detection of reverse transcriptase (RT) activity, we have verified the retroviral origin of the retrovirus-like particles that are produced in very low amounts by the MS cell lines.