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Artifacts in sensitivity-enhanced HSQC.

C J Turner1, P J Connolly, A S Stern

  • 1Francis Bitter Magnet Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA. TURNER@CCNMR.MIT.EDU

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|March 4, 1999
PubMed
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Proton-proton coupling causes artifacts in sensitivity-enhanced HSQC spectra, appearing as remote proton cross-peaks. These artifacts, from relayed coherence transfer, can exceed 10% of the main peak intensity.

Area of Science:

  • Nuclear Magnetic Resonance Spectroscopy
  • Analytical Chemistry

Background:

  • Sensitivity-enhanced HSQC (2D HSQC) is a vital NMR technique.
  • Artifacts can complicate spectral interpretation and quantitative analysis.

Purpose of the Study:

  • To investigate the origin and characteristics of proton-proton coupling artifacts in sensitivity-enhanced HSQC spectra.
  • To quantify the magnitude of these artifacts.

Main Methods:

  • Product operator analysis to theoretically model artifact formation.
  • Experimental validation using 2D NMR spectroscopy.

Main Results:

  • Proton-proton coupling leads to artifacts via relayed coherence transfer during back-transfer.
  • These artifacts manifest as cross-peaks involving remote protons.

Related Experiment Videos

  • Artifact magnitude can exceed 10% of the primary peak signal.

    • Conclusions:

    • Understanding artifact formation is crucial for accurate HSQC spectral interpretation.
    • Relayed coherence transfer is a key mechanism for artifact generation in this technique.
    • The significant magnitude of these artifacts necessitates careful consideration in quantitative NMR studies.