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Related Experiment Videos

MAP5 expression in proliferating neuroblasts.

A Cheng1, B K Krueger, L L Bambrick

  • 1Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore, MD 21201, USA.

Brain Research. Developmental Brain Research
|March 5, 1999
PubMed
Summary

Microtubule-associated protein 5 (MAP5) may be an early marker for proliferating neuronal progenitors in the embryonic mouse brain. Its expression near cell division suggests a role in neuronal commitment.

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • The embryonic mouse telencephalic ventricular zone (VZ) is crucial for generating cortical neurons.
  • Microtubule-associated protein 5 (MAP5) is found in immature neurons and its expression in the VZ was noted.

Purpose of the Study:

  • To investigate if MAP5 is expressed by proliferating neuronal progenitors in the embryonic mouse brain.
  • To determine the timing of MAP5 expression relative to the cell cycle and neuronal commitment.

Main Methods:

  • Used dissociated cortical cells in vitro.
  • Measured MAP5 and nestin expression (a neuroepithelial marker).
  • Assessed bromodeoxyuridine (BrdU) incorporation to identify proliferating cells.

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Main Results:

  • All proliferating cells expressed nestin.
  • A subset of MAP5-positive cells incorporated BrdU, indicating proliferation.
  • MAP5 expression was detected near the time of cell division and commitment to neuronal fate.

Conclusions:

  • MAP5 expression appears shortly before a cell commits to becoming a postmitotic neuron.
  • MAP5 may serve as an early marker for identifying neuronal progenitors destined for terminal differentiation.
  • This finding advances understanding of early cortical development and neuronal lineage commitment.