Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

MAP5 expression in proliferating neuroblasts.

A Cheng1, B K Krueger, L L Bambrick

  • 1Department of Physiology, University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore, MD 21201, USA.

Brain Research. Developmental Brain Research
|March 5, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building better conversations: results of a community-based online health misinformation and motivational interviewing training program in Alaska.

BMC public health·2026
Same author

Trans-Tasman Comparison of Microbial Trends and Antibiotic Resistance Patterns in Severe Odontogenic Infection: A 5-Year Retrospective Analysis of South Australia, Australia and Auckland, New Zealand.

Australian dental journal·2026
Same author

Importance of surveillance and vaccination in managing respiratory syncytial virus infections among older adults in Hong Kong.

Hong Kong medical journal = Xianggang yi xue za zhi·2025
Same author

Author Correction: Evidence for multi-fragmentation and mass shedding of boulders on rubble-pile binary asteroid system (65803) Didymos.

Nature communications·2024
Same author

Evidence for multi-fragmentation and mass shedding of boulders on rubble-pile binary asteroid system (65803) Didymos.

Nature communications·2024
Same author

Plasma electron acceleration driven by a long-wave-infrared laser.

Nature communications·2024
Same journal

Postnatal development of GFAP, connexin43 and connexin30 in cat visual cortex.

Brain research. Developmental brain research·2005
Same journal

Expression of gamma-aminobutyric acid B receptor subunits in hypothalamus of male and female developing rats.

Brain research. Developmental brain research·2005
Same journal

Retinal neurospheres prepared as tissue for transplantation.

Brain research. Developmental brain research·2005
Same journal

Effects of prenatal stress on anxiety and social interactions in adult rats.

Brain research. Developmental brain research·2005
Same journal

Disruption of cerebellar granule cell development in the Pax6 mutant, Sey mouse.

Brain research. Developmental brain research·2005
Same journal

Cytoskeletal, synaptic, and nuclear protein changes associated with rat interface organotypic hippocampal slice culture development.

Brain research. Developmental brain research·2005
See all related articles

Microtubule-associated protein 5 (MAP5) may be an early marker for proliferating neuronal progenitors in the embryonic mouse brain. Its expression near cell division suggests a role in neuronal commitment.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • The embryonic mouse telencephalic ventricular zone (VZ) is crucial for generating cortical neurons.
  • Microtubule-associated protein 5 (MAP5) is found in immature neurons and its expression in the VZ was noted.

Purpose of the Study:

  • To investigate if MAP5 is expressed by proliferating neuronal progenitors in the embryonic mouse brain.
  • To determine the timing of MAP5 expression relative to the cell cycle and neuronal commitment.

Main Methods:

  • Used dissociated cortical cells in vitro.
  • Measured MAP5 and nestin expression (a neuroepithelial marker).
  • Assessed bromodeoxyuridine (BrdU) incorporation to identify proliferating cells.

Related Experiment Videos

Main Results:

  • All proliferating cells expressed nestin.
  • A subset of MAP5-positive cells incorporated BrdU, indicating proliferation.
  • MAP5 expression was detected near the time of cell division and commitment to neuronal fate.

Conclusions:

  • MAP5 expression appears shortly before a cell commits to becoming a postmitotic neuron.
  • MAP5 may serve as an early marker for identifying neuronal progenitors destined for terminal differentiation.
  • This finding advances understanding of early cortical development and neuronal lineage commitment.