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Interaction of Et2SnCl2 with 5'-IMP and 5'-GMP
Z Yang1, T Bakas, A Sanchez-Diaz
1University of Ioannina, Laboratory of Inorganic and General Chemistry, Department of Chemistry, Greece.
Journal of Inorganic Biochemistry
|March 5, 1999
Summary
Diethyltin dichloride (Et2SnCl2) interactions with 5'-IMP and 5'-GMP depend on pH. At low pH, Sn(IV) binds to pyrophosphate; at high pH, it binds to sugar atoms, forming specific solid adducts.
Area of Science:
- Inorganic Chemistry
- Bioinorganic Chemistry
- Coordination Chemistry
Background:
- Nucleotides like 5 eal-inosinic acid (5 eal-IMP) and 5 eal-guanylic acid (5 eal-GMP) are crucial in biological systems.
- Organotin compounds, such as diethyltin dichloride (Et2SnCl2), have diverse applications and biological relevance.
- Understanding metal-nucleotide interactions is key to developing new therapeutic agents and understanding biological processes.
Purpose of the Study:
- To investigate the pH-dependent interactions between diethyltin dichloride (Et2SnCl2) and the nucleotides 5 eal-IMP and 5 eal-GMP in aqueous solutions.
- To characterize the structure and bonding modes of the resulting solid adducts.
- To elucidate the coordination chemistry of Sn(IV) with these biologically relevant molecules.
Main Methods:
- Proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy.
- Phosphorus-31 Nuclear Magnetic Resonance (31P-NMR) spectroscopy.
- Infrared (IR) spectroscopy, solid-state NMR (13C, 31P), 119Sn Mössbauer spectroscopy, and solution 119Sn-NMR spectroscopy.
Main Results:
- At low pH (< 4.0), Et2SnCl2 coordinates with the pyrophosphate groups of 5 eal-IMP and 5 eal-GMP.
- At intermediate pH (4-9.5), no significant interaction between Et2SnCl2 and the nucleotides was observed.
- At high pH (> 9.5), Et2SnCl2 preferentially binds to the O'2 and O'3 atoms of the sugar moiety.
- Solid adducts were characterized, with elemental analysis confirming formulae like (Et2Sn)2(5 eal-IMP)2(H2O) and (Et2Sn)3(5 eal-GMP)2(OH)2(H2O)2.
- Spectroscopic data confirmed pyrophosphate involvement in bonding at low pH, leading to oligomeric or polymeric structures with trigonal bipyramidal or octahedral Sn(IV) geometries.
Conclusions:
- The coordination mode of Et2SnCl2 with 5 eal-IMP and 5 eal-GMP is strongly pH-dependent.
- Pyrophosphate and sugar hydroxyl groups serve as primary binding sites for Sn(IV) at different pH ranges.
- The formation of specific solid adducts and the observed coordination geometries provide insights into the bioinorganic chemistry of organotin-nucleotide interactions.