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Pregnancy following transfer of ooplasm from cryopreserved-thawed donor oocytes into recipient oocytes
S E Lanzendorf1, J F Mayer, J Toner
1The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23507, USA. susanl@jones1.evms.edu
Objective:
To determine if frozen-thawed donor oocytes could be used to provide cytoplasm for transfer into patients' oocytes to improve subsequent embryonic development.
Design:
Prospective evaluation of the procedure in consenting IVF patients.
Setting:
Assisted reproductive technology program.
Patient(S):
The study was open to consenting IVF patients (of any age) with a history of poor embryo quality or those couples in which the wife's age was > or = 40 years.
Intervention(S):
Transfer of donor egg cytoplasm from frozen-thawed oocytes into the oocytes of infertile recipients.
Main Outcome Measure(S):
Donor oocyte survival following cryopreservation, fertilization following cytoplasmic transfer into recipient oocytes, embryo quality, and pregnancy outcome.
Result(S):
Oocytes collected from four donors were cryopreserved and 61% (28/46) survived the thaw procedure. Cytoplasmic transfer was performed on the eggs of four patients, with fertilization occurring in 70.3% (26/37). Twin pregnancy was established in one patient (35 years of age) with a history of poor embryo quality.
Conclusion(S):
Cryopreserved donor oocytes may provide a source of cytoplasm for transfer into recipient oocytes, eliminating the need for cycle synchronization between donor and infertile patient.