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Related Experiment Videos

Capillary electrochromatography with segmented capillaries for controlling electroosmotic flow.

C Yang1, Z El Rassi

  • 1Department of Chemistry, Oklahoma State University, Stillwater 74078-3071, USA.

Electrophoresis
|March 5, 1999
PubMed
Summary

Segmented capillaries with octadecyl silica (ODS) and bare silica segments control electroosmotic flow (EOF) in capillary electrochromatography (CEC). Adjusting segment length reduces analysis time by accelerating EOF.

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Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) relies on electroosmotic flow (EOF) for mobile phase movement.
  • Controlling EOF is crucial for optimizing separation efficiency and reducing analysis time in CEC.

Purpose of the Study:

  • To investigate the use of segmented capillaries with distinct stationary phases for manipulating EOF in CEC.
  • To determine the impact of segmented capillary design on flow characteristics and analysis time.

Main Methods:

  • Development and utilization of segmented capillaries, with one segment packed with octadecyl silica (ODS) and another with bare silica.
  • Systematic variation of the fractional length of the bare silica (EOF accelerator) segment.
  • Analysis of average flow rates and their relationship with capillary configuration and pore size.

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Main Results:

  • Average flow in segmented capillaries increased linearly with the proportion of the EOF accelerator segment.
  • Analysis time was reduced due to the accelerated EOF.
  • The pore size of the bare silica segment influenced the overall flow.
  • Flow in segmented capillaries showed a degeneration from pure EOF towards viscous flow, exacerbated by retaining frits.

Conclusions:

  • Segmented capillaries offer a method for controlling and manipulating EOF in CEC.
  • The design of segmented capillaries, specifically the length of the EOF accelerator segment, can be optimized to reduce analysis times.
  • The presence of retaining frits contributes to flow degeneration, impacting separation performance in CEC.