Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel method of chronically blocking retinal activity.

G T Prusky1, A S Ramoa

  • 1Department of Psychology and Neuroscience, The University of Lethbridge, Alberta, Canada. prusky@uleth.ca

Journal of Neuroscience Methods
|March 5, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Treatment of age-related visual impairment with a peptide acting on mitochondria.

Disease models & mechanisms·2021
Same author

Perception of visual motion coherence by rats and mice.

Vision research·2006
Same author

Independent visual threshold measurements in the two eyes of freely moving rats and mice using a virtual-reality optokinetic system.

Visual neuroscience·2005
Same author

Characterization of mouse cortical spatial vision.

Vision research·2004
Same author

Preservation of vision following cell-based therapies in a model of retinal degenerative disease.

Vision research·2004
Same author

Visual memory task for rats reveals an essential role for hippocampus and perirhinal cortex.

Proceedings of the National Academy of Sciences of the United States of America·2004
Same journal

Detection of cochlear microphonic for differential diagnosis between auditory neuropathy mice and noise-induced sensorineural hearing loss mice.

Journal of neuroscience methods·2026
Same journal

Assessment metrics for pain control in rats: A methodological commentary.

Journal of neuroscience methods·2026
Same journal

Infant EEG preprocessing pipelines: A capability framework and current gaps in practice.

Journal of neuroscience methods·2026
Same journal

Methods for measuring neural activity during voluntary wheel running.

Journal of neuroscience methods·2026
Same journal

Serotype-dependent differences in AAV cellular transduction rates in the hypothalamus of Arctic ground squirrels.

Journal of neuroscience methods·2026
Same journal

Rapid generation of human sensory neurons from iPSC for modeling of peripheral neuropathies.

Journal of neuroscience methods·2026
See all related articles

Ethylene-vinyl acetate copolymer Elvax implants containing tetrodotoxin (TTX) effectively blocked ferret retinal activity for 25 days. This novel method offers a sustained, non-damaging alternative to repeated TTX injections for blocking retinal function.

Area of Science:

  • Neuroscience
  • Ophthalmology
  • Biomaterials Science

Background:

  • Ethylene-vinyl acetate copolymer (Elvax) is a known vehicle for delivering bioactive substances to the nervous system.
  • Chronic blocking of retinal activity is crucial for studying visual pathways and developing treatments for retinal disorders.

Purpose of the Study:

  • To investigate the efficacy of Elvax implants delivering tetrodotoxin (TTX) for sustained, chronic blockade of retinal activity.
  • To evaluate the safety and duration of Elvax-mediated TTX delivery in the vitreous humor of ferret eyes.

Main Methods:

  • Surgically implanting small Elvax pieces loaded with tetrodotoxin (TTX) into the vitreous humor of ferret eyes.
  • Monitoring the light-induced pupillary reflex to assess visual pathway function.

Related Experiment Videos

  • Conducting electrophysiological assays of the vitreous humor to confirm retinal activity blockade.

    • Main Results:

    • Elvax implants successfully and completely blocked retinal activity for up to 25 days.
    • No apparent retinal damage was observed during the study period.
    • The pupillary reflex and electrophysiological data confirmed sustained functional blockade.

    Conclusions:

    • Elvax implants provide a novel, effective, and safe method for chronic retinal activity blockade in experimental models.
    • This technique offers significant advantages over frequent injections, reducing animal handling and improving experimental consistency.
    • The sustained blockade capability opens new avenues for research in visual neuroscience and ophthalmology.