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Related Experiment Videos

Microbial genome sequencing and pathogenesis.

C M Tang1, D W Hood, E R Moxon

  • 1Molecular Infectious Diseases Group, University Department of Paediatrics, Oxford University, John Radcliffe Hospital, Oxford OX3 9DU, UK. christophtang@paediatrics.oxford.ac.uk

Current Opinion in Microbiology
|March 6, 1999
PubMed
Summary
This summary is machine-generated.

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Genome sequencing projects for major bacterial pathogens are nearing completion. While database alignments reveal nucleotide differences, many open reading frames lack known functions, with experimental validation just beginning.

Area of Science:

  • Microbiology
  • Genomics
  • Bioinformatics

Background:

  • The late 1990s marked a milestone with the complete nucleotide sequencing of Helicobacter pylori and Escherichia coli.
  • Anticipation grew for the complete genome sequences of all major human bacterial pathogens by the end of the century.

Purpose of the Study:

  • To analyze sequenced bacterial genomes using database alignments.
  • To assign putative functions to open reading frames (ORFs).
  • To identify nucleotide-level differences between bacterial species.

Main Methods:

  • Utilizing database alignments for comparative genomics.
  • Analyzing open reading frames (ORFs) within sequenced bacterial genomes.
  • Comparing nucleotide sequences to differentiate bacterial species.

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Main Results:

  • The high proportion of open reading frames with unknown functions emerged as a significant finding across genome projects.
  • Database alignments facilitated the assignment of putative functions to numerous ORFs.
  • Nucleotide-level comparisons highlighted inter-species variations.

Conclusions:

  • Genome sequencing projects provide a foundational resource for understanding bacterial pathogens.
  • The identification of ORFs with unknown functions underscores the need for further experimental investigation.
  • Experimental data validating the utility of these genome sequencing projects is emerging.