Bacterial solute uptake and efflux systems
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Updated: Oct 24, 2026

High-Throughput Expression and Purification of Human Solute Carriers for Structural and Biochemical Studies
Published on: September 29, 2023
J S Lolkema1, B Poolman, W N Konings
1Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands. j.s.lolkema@biol.rug.nl
Abstract:
The recent discovery of binding protein dependent secondary transporters and the ever-growing family of membrane potential generating secondary transporters emphasize the diversity of transport systems in both the mechanistical and physiological sense. The vast amount of data on the lactose permease is now beginning to crystallize in a model that relates functional events to structural changes of the protein. Evidence has been presented that multidrug transporters pick up their substrates from the membrane, and the binding of a number of substrates to the binding-protein components of ATP-driven transporters is now understood in detail.
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