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Published on: September 15, 2023
Green fluorescent protein as a reporter of transcription and protein localization in fungi
1Department of Molecular Biology, Johns Hopkins Medical School, Preclinical Teaching Building 522, 725 North Wolfe Street, Baltimore MD 21205-2185, USA. bcormack@bs.jhmi.edu
Abstract:
Green fluorescent protein (GFP) is a versatile and powerful tool for analysis of diverse biological processes. The recent development of GFP variants with altered spectral properties and altered codon composition has allowed efficient expression of GFP in a number of fungal species. GFP has been successfully used to analyze transcription regulation as well as protein and organelle localization, and promises to give an unprecedented view into the dynamic subcellular processes that shape the fungal cell.
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