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Related Experiment Videos

Advances in cereal gene transfer.

T Komari1, Y Hiei, Y Ishida

  • 1Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., 700 Higashibara, Toyoda, Iwata, Shizuoka 438-0802, Japan. Toshihiko. Komari@pbgrl.jti.co.jp

Current Opinion in Plant Biology
|March 6, 1999
PubMed
Summary

Transgenic cereal production has advanced, with rice and maize transformation being common. Agrobacterium-mediated transformation is emerging as the preferred method over particle bombardment for creating genetically modified crops.

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Plant molecular biology·2000

Area of Science:

  • Plant biotechnology
  • Genetics and genomics
  • Agricultural science

Background:

  • Significant advancements in creating transgenic cereal strains over the last five years.
  • Rice and maize are the most frequently targeted cereal crops for genetic modification.
  • Particle bombardment has been the predominant technique for generating cereal transformants.

Purpose of the Study:

  • To review the current state of cereal transformation technologies.
  • To highlight the shift towards Agrobacterium-mediated transformation.
  • To establish rice as a model monocot for transformation studies.

Main Methods:

  • Review of published literature on cereal transformation techniques.
  • Analysis of transformation efficiencies and applications.

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  • Focus on particle bombardment and Agrobacterium-mediated methods.

    • Main Results:

    • Widespread production of transgenic strains in major cereals.
    • Particle bombardment historically dominant, but Agrobacterium-mediated transformation gaining prominence.
    • Rice exhibits the most advanced transformation technology among monocots.

    Conclusions:

    • Agrobacterium-mediated transformation is becoming the standard for cereal modification.
    • Rice serves as a key model organism for advancing monocot transformation research.
    • Continued development in transformation techniques will impact crop improvement.