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Overexpression of the FNR protein of Escherichia coli with T7 expression system
1Department of Molecular Biology, Comenius University, Bratislava, Slovakia. stuchlik@fns.uniba.sk
Folia Microbiologica
|March 9, 1999
Abstract:
We have used the T7 expression system for expression of E. coli FNR protein. The fnr gene was cloned from its initiation codon ATG into the NdeI site of an expression vector and filamentous phage mGP1-2 was used as a donor of T7 RNA polymerase gene. The level of FNR expression attained by this expression arrangement was about 45% of total cell proteins.