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Autoreactive human T cell lines recognizing ribosomal protein L7.

J Donauer1, M Wochner, E Witte

  • 1Faculty of Biology, University of Konstanz, Germany.

International Immunology
|March 9, 1999
PubMed
Summary
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Systemic lupus erythematosus (SLE) patients

Area of Science:

  • Immunology
  • Autoimmunity
  • Molecular Biology

Background:

  • Systemic lupus erythematosus (SLE) is characterized by autoantibodies, including those targeting ribosomal protein L7 (rpL7).
  • The autoimmune response to rpL7 involves both B cells and T cells.
  • Understanding T cell responses is crucial for elucidating SLE pathogenesis.

Purpose of the Study:

  • To investigate the T cell response to ribosomal protein L7 (rpL7) in systemic lupus erythematosus (SLE).
  • To characterize the phenotype and specificity of rpL7-reactive T cells.

Main Methods:

  • Peripheral blood lymphocytes from SLE patients and healthy donors were cultured with recombinant rpL7.
  • Cytokine secretion (IFN-gamma, IL-4, IL-10) was measured using spot-ELISA.
  • rpL7-reactive T cell lines were established via limiting dilution cloning and characterized (phenotype, TCR, HLA restriction).

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Main Results:

  • T cells from SLE patients and healthy donors showed increased IFN-gamma secretion upon rpL7 stimulation.
  • Six rpL7-reactive, IFN-gamma-secreting T cell lines with a CD4+ phenotype were established.
  • Specific rpL7 epitopes and HLA-DR/DP restriction were identified for some T cell lines.

Conclusions:

  • T cells from SLE patients and healthy individuals can recognize ribosomal protein L7 (rpL7).
  • The T cell response to rpL7 is characterized by IFN-gamma secretion and specific HLA restriction.
  • These findings contribute to understanding the T cell-mediated autoimmune response in SLE.